General Cell Based Assays
Eurofins Discoverx cAMP Hunter GPBAR1 Bioassay Kit, 2-Plate. (95-0049Y2-00139)
The cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a high-throughput 384-well format. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter™ Human PPYR1 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human PPYR1 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PPYR1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human GHRHR Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human GHRHR Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GHRHR (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ABclonal Technology HRP-conjugated B-Actin Mouse
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
ABclonal Technology GCGR Rabbit pAb
The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).
Eurofins Discoverx PathHunter Human F2RL1 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human F2RL1 β-Arrestin Stable Cell Line Assay (HEK 293) β-Arrestin Cell Line contains a stable clonal cell line used to measure F2RL1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CCRL2 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0333E2ACP1M)
The PathHunter CCRL2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCRL2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCRL2 and EA-tagged β-Arrestin-2. Activation of CCRL2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical THUNDERPhosphop38a MAPK 480
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD4(1) Bromodomain Assay, 10-plate Kit. (96-0005E1CP0L)
The InCELL Hunter eXpress HEK 293 BRD4(1) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD4(1) via detection of BRD4(1) levels. This product uses EFC technology. The cell line included overexpresses BRD4(1) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical MitoLite™ Orange FX570, 500 test
A mitochondrial dye for live and fixed cells; ex/em = 545/575 nm; has been used to label mitochondria in Hep2 cells treated with cisplatin and NAC
Enzo Life Sciences LYSO-ID Red cytotoxicity kit (GFP-CERTIFIED) (1Kit)
The LYSO-ID Red Cytotoxicity Kit monitors dysfunction of lysosomal degradation using a drug-like cationic amphiphilic tracer (CAT) dye that rapidly and selectively stains acidic organelles, and is suitable for monitoring accumulation of lysosomes and lysosome-like structures in live cells. Mechanism of Action A cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. This tracer was created by placing titratable groups on the probe (dye), to enable labeling to be expanded into lamellar inclusion bodies of cells pretreated with weakly basic cell-permeant compounds. Besides lysosomes themselves, the probe can be employed for highlighting lysosome-like organelles under conditions wherein cells produce vacuoles that contain most of the degradative enzymes of the lysosome but are not as acidic as the parent organelle. Applications: Fluorescence microscopy, Fluorescent detection .
Eurofins Discoverx cAMP Hunter™ Human TSHR(L) Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human TSHR(L) Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure TSHRL (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eagle Biosciences Inc Nitric Oxide Synthase Non-Enzymatic Assay Kit
Nitric Oxide Synthase Non-Enzymatic Assay Kit
Eurofins Discoverx cAMP Hunter eXpress VIPR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0052E2CP2L)
The cAMP Hunter VIPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type VIPR2, a naturally Gs coupled GPCR. Upon activation of VIPR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Phospho-IR-β (Insulin Receptor Beta) (Tyr1150 /1151) Detection Kit, 500 Assay Points
HTRF Phospho-IR-β (Insulin Receptor Beta) (Tyr1150 /1151) Detection Kit, 500 Assay Points